Reporter

Part:BBa_K274003:Design

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-10-18)


Vio operon ABDE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5239
    Illegal SapI.rc site found at 5314


Design Notes

The origonal vio operon was designed with the vioC gene flanked by complementary sticky end restriction sites for BamHI and BglII. This allowed the vioC to be easily removed, leading to the production of green pigment.


Source

This part was made from the vio operon biobrick, part number BBa_K274002. The vioC gene has been removed by restriction digest.

References